Multilayered habitation sites are very important in our understanding of chronological framework and building the regional periodization of ancient cultures. It allows us to reconstruct the relative and chronometric timing of past events, and therefore, should become a priority in the archaeological investigations. Olkhon (Little Sea) region is considered to be one of the potential geoarcheological regions to use for studying the cultural remains of Holocene in the Cis-Baikal region of Eastern Siberia. This region contains only a few multilayered habitation sites, and site Kulara III is one of them. This article is devoted to the analysis of all material recovered at this location dated to the Early and Middle Holocene period 11 260–8280 cal. BP and 6180–5990 cal. BP. This paper includes description of zooarchaeological remains and new radiocarbon dates made on the organic components of sediments. Despite the low numbers of archaeological materials recovered during the excavations of habitation site Kulara III, it provides good record of Early and Middle Holocene deposits, which provide opportunity for its comparison with other habitation sites in the Olkhon region.